Objectives: Biofilms represent the most common type of microbial growth in nature and are critical to development of many infections in human. Recently it has been reported that 65% of all human infections involve biofilms. Nowadays Candida albicans has more effective role among nosocomial pathogens due to proper potential for biofilm formation. Using a novel method in field of inhibitory formation of C. albicans biofilm and elimination of fungal mass over surfaces is valuable for controlling infections. In this study antifungal effect of ZnO/Ag nanoparticles were evaluated on standard strain of C. albicans. Methods: ZnO nanoparticles and Ag were synthesized through the solgel method by Zinc Acetate Dehydrate [Zn(CH3COOH)2.2H2O] and AgNo3 as precursor respectively. The morphology properties of the products were analyzed by using Scanning Electron Microscopy (SEM). Biofilm of standard strain of C. albicans (ATCC 10231) was developed on flat-bottomed 96-well microtiter plates and antifungal effects of ZnO/Ag nanoparticles were evaluated by using standard accurate method, CCK8 (Cell Counting Kit 8) as colorimetric assay for measuring metabolic activity of sessile cells within the biofilm as it relates to cellular viability. Data were analyzed using t-test statistical method and SPSS software.
Full conference title:
22nd European Congress of Clinical Microbiology and Infectious Diseases
- ECCMID 22nd (2012)