EUCAST Broth Dilution Method: In Vitro Activities of Currently Available Antifungal Agents against Filamentous Fungi.

C. LASS-FLOERL1, S. PERKHOFER 1, M. CUENCA-ESTRELLA 2;

Author address: 

1Inst. of Hygiene and Social Med., Innsbruck, Austria, 2Servicio de Micologica, Madrid, Spain.

Abstract: 

Background: The European Subcommittee on Antifungal Susceptibility Testing prepared a broth dilution method for Aspergillus and moulds (proposed EUCAST-ASP). Methods: We determined MICs of amphotericin B (AMB), liposomal amphotericin (l-AMB), itraconazole (ITZ), voriconazole (VRZ), posaconazole (POS) and caspofungin (CAS) against Aspergillus fumgitus (n=26), Aspergillus terreus (n=19), Aspergillus niger (n=8), Aspergillus flavus (n=16), Penicillium spp. (n=4), Fusarium spp. (n=7), Rhizopus spp. (n=12), Absidia spp. (n=13) and Mucor spp. (n=12) according to EUCAST-ASP. Results: The MIC 90% against Aspergillus spp. (not A. terreus) were 1 mg/L for AMB, l-AMB, 2 mg/L for ITZ, 0.5 mg/L for VRZ, POS and CAS, respectively. The MIC 90% against A. terreus was 4 mg/L for AMB, l-AMB, 2 mg/L for ITZ, 0.5 mg/L for VRZ, POS and CAS, respectively. The MIC 90% against Penicillium spp. were 2 mg/L for AMB, l-AMB, and POS, > 8 mg/L for VRZ, ITZ and CAS, respectively. For all zygomyzetes the MICs 90% were 2 mg/L for AMB, l-AMB, 4 mg/L for POS, >8 mg/L for ITZ, VRZ, and CAS, respectively. For all Fusarium spp. the MICs 90% were 4 mg/L for AMB, l-AMB, >8 mg/L for POS, ITZ, VRZ, and CAS, respectively. Conclusion: MICs obtained by the EUCAST antifungal susceptibility testing methods are comparable to those found by others. MICs for the four AMB-formulations were similar, showing no influences on the various AMB-drug preparations. AMB and POS were found to be active against the majority of pathogenic fungi. Hyaline filamentous fungi and zygomyzetes appear to be very heterogenous in their in vitro susceptibilities: azoles are not active against Fusarium spp. yet polyenes; Mucor spp. seems to be in vitro resistant against POS, Rhizopus spp. is not.
2007

abstract No: 

M-543

Full conference title: 

47th Interscience Conference on Antimicrobial agents and Chemotherapy
    • ICAAC 47th