Establishment of novel model of onychomycosis in rabbits for evaluation of antifungal agents

Shimamura T., Kubota N., Nagasaka S., Suzuki T., Mukai H., Shibuya K.

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It has been reported that animal models of onychomycosis were used to evaluate many new drugs; the pathophysiology of experimental infection in rodent however has been obscure. In the study, we report an establishment of novel animal model using rabbit of which excellent reproducibility was confirmed. In addition, it was also confirmed that pathophysiology of the model accurately reflect that of a particular type of onychomycosis in human, subungal type. Finally we evaluated some antifungals using our model with mycological and histopathological exainations. Trichophyton mentagrophytes (TIMM2789) were infected to the nail of hind limb in the Japanese white rabbit under the steroid treatment for 2 or 4 weeks as their nails were made moistened by latex glove. After treatment with infection, these nails in rabbits were observed for 0, 2, 4 and 6 weeks to check the change of condition. Then, these nails were taken from the feet, and they were evaluated the localization of fungus in the tissue sample after staining with periodic acid- Schiff stain. In the result, the onychomycosis model using rabbit were established under the condition which microconidia was infected for 2 weeks. Organism was confirmed in the nail plate and nail bed side in the nail, and infection rate in this model was over 86.7%. Additionally, fungi increased into the nail and were confirmed to the localization into the nail. Moreover, Penlac, as an antifungal drug, was applied to the nail for 4 weeks, and was confirmed the efficacy. In conclusion, we established the onychomycosis model which it was useful for the evaluation of the antifungal efficacy.

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17th International Society for Human and Animal Mycology
    • ISHAM 17th (2009)