Purpose: Genomic resources for studying the biology and pathology of A. fumigatus are limited in two critical ways. The first limitation is that we have available the genome sequences of only two strains, Af293 and A1163. The low quality of the genome annotation of A. fumigatus is the second major limitation to studies on A. fumigatus. Methods: To address these limitations, we will sequence de-novo two additional strains (Af210 and Af10) carefully selected to provide a much broader representation of the species variation. In addition, we will generate reference-guided genome assemblies for 42 drug resistant strains using the Illumina short-read sequencing technology. Results: In addition, preliminary RNA-Seq data has been generated to quantify gene expression for the entire A. fumigatus transcriptome. Gene expression data from a time course experiment using the laeA mutant confirmed the role of glitoxin in the neutropenic mouse infection model. Simultaneously, the host immune response in neutropenic mice infected with deltalaeA was measured using the SuperArray RT-PCR assay. The assay demonstrated that 11 genes including T2bp, Tlr4, Cd14, and Myd88, which activate the NF-KB pathway, are up-expressed in deltalaeA compared to the wild type. Conclusions: This is consistent with gliotoxin-mediated NF-KB inhibition observed during aspergillosis.
Full conference title:
4th Advances Against Aspergillosis
- AAA 4th (2010)