Enhancement of antimicrobial activity produced by Aspergillus fumigatus.

Niege Araçari Jacometti Cardoso Furtado, Suraia Said, Izabel Yoko Ito, Jairo Kenupp Bastos

Author address: 

Farmacëuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brasil.


In the course of a screening program for new antibiotic producers, a strain of Aspergillus fumigatus was isolated from Brazilian soil samples (Pantanal, MS). This fungus was first grown on seed medium to achieve biomass and after 24 hours it was transferred to a production medium. A pool of living or autoclaved microorganisms was added or not to the fungus culture on the second day in the production medium at the ratio of 1:6 to increase antibiotic production. After different times the culture broths were separated from mycelium and extracted with chloroform and butanol. Extracts were analyzed by HPLC and submitted to antimicrobial activity evaluation by agar diffusion assay and bioautography. There was a 55% increase in activity against Staphylococcus aureus, a 63% increase against Candida albicans and more than 100% against Micrococcus luteus in chloroform extracts from the broth of cultures in which the pool of living or autoclaved microorganisms was present. The chromatographic profiles of chloroform extracts from the two culture conditions were different. By bioautography two additional active compounds were detected in chloroform extract from the broth of cultures grown in the presence of pooled microorganisms and 1,3,5-trimethoxybenzene and 2,5-dimethoxyphenol were identified in this extract. Supported by FAPESP (Proc. n . 99/09850-8

abstract No: 


Full conference title: 

21st Fungal Genetics Conference
    • Fungal Genetics Conference 21st (2000)