Enhance itaconic acid production in Aspergillus via cultivation condition optimization

An Li, Nina Pfelzer, Robbert Zuijderwijk, Anja Brickwedde, Peter J. Punt

Author address: 

TNO Microbiology & Systems Biology ,  Hochschule Bremen  , Leiden University


The black filamentous fungi Aspergillus niger has a long tradition of safe use in the production of enzymes and organic acids, and is widely used in biotechnology as host for the production of food ingredients, pharmaceuticals and industrial enzymes. Besides, Aspergillus niger grows on a wide range of substrates under various environmental conditions. In our research we have addressed the production of one of the commercially interesting building8208;block organic acids, itaconic acid. The transcriptomics analysis from A. terreus identified the most relevant itaconic acid related genes. Subsequently, expressing the specific cis8208;aconitate decarboxylase (CAD) encoding gene in A niger lead to itaconic acid production in the fermentation medium. To enhance itaconic acid production level via medium improvement, 20 different media were designed based on a reference medium from A. terreus and a 968208;well micro8208;titer plate screening assay was applied for screening . The best medium increased the production level in controlled batch fermentation. This was confirmed showing that various levels of one of the trace elements correlated with itaconic acid production under these conditions. In addition, several other parameters such as pH, temperature and dissolved oxygen tension (D.O.) in controlled batch fermentations were shown to be important for itaconic acid production .

abstract No: 


Full conference title: 

11 th European Conference on Fungal Genetics
    • ECFG 11th (2012)