Engineering intracellular metabolism by altering gene expression of Aspergillus oryzae

Hideaki Koike 1, Tomomi Toda 1, Tomoko Ishii 1, Junichiro M arui 1, Sumiko Ohashi1, Hiroko Hagiwara 1, Kenichiro M atsushima 2, Tadashi Takahashi2, Akira Ohyama 3, Motoaki Sano 4, Shinichi Ohashi4, Marie Nishimura 5, Yasuji Koyama 2 and Masayuki Mac

Author address: 

1 Natl. Inst. Adv. Indus. Sci. Tech. (AIST), 2 Noda Inst. Sci. Res., 3 Insilico Biology, 4 Kanazawa Inst. Tech., 5 Natl. Inst. Agrobiol. Sci.


Aspergillus oryzae is one of the most important organisms in Japanese fermented food industry. Though it hardly produces secondary metabolites, related organisms are producers of diverse metabolites. A major objective of our project is to develop a system using A. oryzae to generate diverse metabolites. Combinatorial biochemistry approach by introducing exo-genous genes and fermentation techniques are combined. To reveal regulation of metabolic pathways in A. oryzae under various conditions, gene expression profiles under condition of submerged culture, solid media and so on were analyzed by DNA micro array. A series of deletion mutants of transcription factor have been obtained by systematic gene targeting. More than 200 mutants were tested for their ability to produce some metabolites. Several mutants showed lack of production or different dependency on environmental nutrient conditions. The factors affecting expression level of metabolic genes are being studied. To develop the system, novel vectors and host strains of A. oryzae have been constructed. W e have replaced promoter regions of some metabolic genes and successfully altered the level of some metabolite productions. To introduce genes, the correct detection of splicing position is important. W e have analyzed motifs of splicing in A. oryzae by comparing EST and genome sequences.

abstract No: 


Full conference title: 

6th International Aspergillus Meeting
    • Asperfest 6 (2009)