Effects of Voriconazole (VCZ) and Anidulafungin (ANID) on Flow Culture Biofilms of C. glabrata (Cg) and C. parapsilosis (Cp).

H. BERNHARDT1, M. KNOKE 1, G. SCHWESINGER 1, A. GLOECKNER 2, J. BUFLER 3, V. CZAIKA 4, K. LUDWIG 5, J. BERNHARDT 5;

Author address: 

1Univ. of Greifswald, Greifswald, Germany, 2NRZ, Greifswald, Germany, 3Pfizer Pharma GmbH, Karlsruhe, Germany, 4Helios Clin., Bad Saarow, Germany, 5Clinical Ctr. Suedstadt, Rostock, Germany.

Abstract: 

Background: We investigated the effect of VCZ and ANID on biofilms of Cg and Cp in a miniature flow cell system. Methods: 2 blood culture isolates of Cg and Cp each were grown in flow cells. Cultures were incubated +/- VCZ (16 mg/L) or ANID (8 or 32 mg/L). Biomass, glucose levels, pH and planktonic CFUs were measured. VCZ MICs were determined using DIN and Etest® methods on RPMI or Mueller-Hinton methylene blue agar. Self-made diffusion disks were used for ANID. Results: All Candida strains established biofilms after 24-36 h. 2 days after adding VCZ, the biomass of Cg decreased and glucose levels increased. Small amounts of biomass were produced after prolonged incubation. Planktonic Cg was recovered at 104 cfu/mL vs. 106 cfu/mL (controls). MICs of biofilms resuspended on day 7 were 0.5-1 µg/mL. There was no indication of resistance. In ANID experiments, glucose metabolism ceased within 24 h and no biomass remained after 3-4 d. Planktonic cell counts dropped from 107 to
2007

abstract No: 

M-1845

Full conference title: 

47th Interscience Conference on Antimicrobial agents and Chemotherapy
    • ICAAC 47th