Effects of Human Polymorphonuclear Neutrophils (PMNs) Alone or in Combination with Caspofungin (CAS) Against Candida Albicans Biofilms.

A. KATRAGKOU, M. SIMITSOPOULOU, M. DALAKIOURIDOU, T. KONSTANTINOU, E. DIZA-MATAFTSI, C. TSANTALI, E. ROILIDES;

Author address: 

Aristotle Univ. of Thessaloniki, Thessaloniki, Greece.

Abstract: 

Background: Candida albicans (CA) forms biofilms (BF) on indwelling medical devices. BF are a niche where fungi are protected from host defenses and antifungal agents. As little is known about the effect of host defenses against CA BF, we sought to determine the activity of PMNs alone and in combination with CAS against CA BF as compared to the free-living planktonic (PL) counterparts. Methods: A CA strain isolated from intravascular catheter (CA-M61) was used. PL cells were grown in YNB at 37ºC overnight. BF were grown on silicone elastomer disks in 96-well plates for 48h at 37ºC. PMNs from healthy donors (at PMN/target ratios 1:1 or 5:1) and CAS (0.0625 or 0.015 mg/l) alone or in combination were incubated with mature BF and PL cells with autologous serum for a further 20h at 37ºC with 5% CO2. Plain BF and PL cells served as growth controls. Damage of BF and PL cells was assessed by an XTT colorimetric micro-assay. Statistical analysis (n=7) included ANOVA and post hoc analysis (P
2006

abstract No: 

M-1768

Full conference title: 

46th Interscience Conference on Antimicrobial Agents and Chemotherapy
    • ICAAC 46th