Effects of Aqueous and Ethanolic Extracts of Heinsia crinita on the Pathogenic Fungi of Carica papaya

E. Enemo, D. Anthony

Author address: 

Univ. of Uyo, Uyo, Nigeria

Abstract: 

Background: The need for edible medicinal sources of antifungal agents has risen in recent times owing to the increasing resistance of pathogenic fungi to synthetic antifungal agents. Hence, this research.Methods: Pawpaw fruits were bought from three different markets in Uyo metropolise(Itam, Use and Akpana Ndem markets). Sterilised surgical blade was used to cut out 3mm by 3mm from the diseased area of the pawpaw fruits. This was inoculated on a Potato Dextrose Agar(PDA) media and incubated at room temperature for 5 days. Observed mixed growths were subcultured to get pure cultures. For the exact identification of the isolates further molecular methods will be employed(which is beyond the scope of this work). The fungi isolates were re-inoculated into healthy matured pawpaw fruits to test their pathogenicity. All the isolates were able to produce spoilage with EKP41 being the most pathogenic, and EKP12 being the least pathogenic. Fungi spores were obtained by growing the isolates in peptone broth. The effects of the extracts were tested on the spores using both Agar Disc Diffusion and Agar Well Diffusion methods at two levels (125mg/ml) and (50mg/ml).Results: Of the seven fungal samples, only EKP41 exhibited slight sensitivity to the extracts.Conclusions: The result confirms Heinsia crinita's antifungal property. Hence, the consumption of Heinsia crinita is recommended for curing fungal infections of pawpaw origin, especially of the EKP41 source.
2016

abstract No: 

SUNDAY-393

Full conference title: 

ASM Microbe 2016
    • ASM microbe 1st (2016)