Soil, cotton fibre and animal dung samples were collected from various locations. The soil plate, soil dilution plate and alcohol treatment methods as well as the soil plate and Gochenaur8223;s glucose ammonium nitrate agar (GAN) were used for isolation of Emericella spp. Macroscopic morphological study of Emericella spp. were recorded as growth rate, growth pattern and colour on standard culture media. Microscopic morphological study was examined as conidial head, conidia, ascospore and hülle cells under light and scanning electron microscopes. Molecular analysis of the fungal strain were employed based on the 61538;-tubulin gene and analyzed with the BLAST search. Nineteen isolates of Emericella belonging to 6 species were found including E. corrugate, E. nidulans, E. purpurea, E. quadrilineata, E. rugulosa and E. variecolor. Nothworthy Emericella representing new records for Thailand are E. corrugate, E. purpurea and E. quadrilineata. In vitro antagonistic test was carried out using 10 isolates of Emericella spp. as dual cultures on PDA against 11 species of plant pathogenic fungi as dual culture on PDA, including Alternaria brassicicola, Curvularia lunata, Colletotrichum capsici, Colletotrichum gloeosporioides, Fusarium oxysporum, Helminthosporium sp., Pestalotiopsis sp., Phytophthora palmivora, Pythium aphanidermatum, Rhizoctonia oryzae and Sclerotium rolfsii. The result revealed that E. corrugate, E. nidulans, E. rugulosa and E. variecolor. could inhibit 45-63% mycelium growth of Alternaria brassicicola, Curvularia lunata, Colletotrichum capsici, Colletotrichum gloeosporioides, Fusarium oxysporum, Helminthosporium sp., Pestalotiopsis sp. and Phytophthora palmivora, whereas all of isolates failed to inhibit mycelial growth of Pythium aphanidermatum and two Basidiomycetes anamorphs, Rhizoctonia oryzae and Sclerotium rolfsii.
Full conference title:
Asian Mycological Congress 2013 and the 13th International Marine and Freshwater Mycology Symposium
- AMC 2013