Distribution and antifungal susceptibility patterns of Candida spp causing bloodstream infections in an French University Hospital during 2003-2006

Paugam, A.P., Baixench, M.T.

Author address: 

Hôpital Cochin, PARIS, France

Abstract: 

Objective: The purpose of this study was to evaluate the prevalence and the sensitivity to antifungal drugs of Candida spp isolated by blood culture from hospitalised patients during the last four years. Material and methods: Bloodstream infections were detected with the continuously monitored blood culture system BacT/Alert® (bioMí©rieux, France). For species identification we used the yeast identification system API-10C (bioMí©rieux, France). For antifungal susceptibility we used the E-tested system (AES Laboratoire, Combourg, France). Results: During 4 years (2003-2006), a total of 100 blood cultures were analysed from 100 candidemic patients. The distribution of Candida species according the years were summarized in table I. During the four-year period of your study, C. albicans, obtained from 50 cultures, was the most frequent species (50%), followed in prevalence by C. parapsilosis (16%), C. glabrata (15%), C. tropicalis (5%), C. krusei (5%), C. guillermondii (3%), C. kefyr (2%), C. lusitaniae (1%), C. dubliniensis (1%), C. utilis (1%) and C. fermantatii (1%). Results of the in vitro susceptibility testing of all the isolates did not show resistance to amphotericin B. Among the different species of Candida resistance to fluconazole (MIC ³ 64 µg/ml) was observed with 5 isolates of C. krusei (100%), one isolate of C. glabrata (7%) and one isolate of C. parapsilosis (6%). Resistance to voriconazole (MIC ³ 4 µg/ml) was observed with one isolate of C. glabrata (7%) which exhibit cross resistance to fluconazole. Conclusions: In our medical centre, over the four-year period C. albicans continues to be the most prevalent isolate from candidemia however, an increasing role of non- albicans spp has been observed (from 41% in 2003 to 53% in 2006). The estimated proportion of C.parapsilosis/non albicans spp observed annually is increasing: 2003 (9%), 2004 (12%), 2005 (36%) and 2006 (50%). This study demonstrated the emergence of candidemia caused by C. parapsilosis in a French university hospital.
2007

abstract No: 

P111

Full conference title: 

3rd Trends in Medical Mycology
    • TIMM 3rd (2011)