Discrimination of sequential Aspergillus fumigatus isolates by ligation-mediated PCR fingerprinting analysis

Marti Ras, Nuria., Luc Dua, Olivier Denis, Fréderique Jacobs and;Nolard., Nicole


A. fumigatus is an ubiquitous airbone opportunistic fungus causing severe infections in immunocompromised individuals. Up to now, several methods have been used to discriminate A. fumigatus strains. Typically, the typing method most currently used relies upon RAPD typing. Other methods based upon restriction fragment length polymorphism have also been developed for typing A. fumigatus. In this study, we investigated the diversity of A. fumigatus isolates recovered sequencially from sputum, over a 2-year-period from the 1 patient with fibrosis cystic. In order to genotype different isolates, we typed A. fumigatus using ligation-mediated PCR (LM-PCR). We took advantage of a retrotransposon-like element Afut1 to amplify specifically the flanking fragment located on the 5’ side of this element. The results indicated that LM-PCR technique is reproductible, easy to perform, and provide sufficient discrimination power for epidemiological studies. Our results suggest that patients are colonized by different A. fumigatus strains and support the persistence over time of single strains.__________

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6th Congress of the European Confederation of Medical Mycology Societies
    • ECMM 6th (2000)