Differential expression of four different chitin synthase genes of Aspergillus nidulans in response to developmental status and environmental stresses.

Jeong Im Lee, Yoo Mi Rho, Min Young Lee, Beom Chan Park, Hee Moon Park, and Pil Jae Maeng.


Aspergillus nidulans has five chs genes encoding chitin synthases. We analyzed the expression mode of the four of the five chitin synthase genes, chsA,chsB, chsC, and chsD, using a mutant version of green fluorescent protein (sGFP) as a vital reporter. chsA was expressed at a considerable level in whole conidiophores during asexual development, but was expressed little in vegetative hyphae and foot cells throughout the culture period. During sexual development, chsA was not expressed either in the mycelia induced to sexual development or in the sexual structures. Expression of chsB was not only ubiquitous throughout the fungal body including substrate mycelia and conidiophores but also regardless of the developmental status of the cells. chsC was expressed only at basal levels during vegetative growth and asexual development. However, during sexual development, chsC was expressed at a considerable level in the shells of young cleistothecia and was strongly expressed specifically in mature ascospores. chsD was expressed at basal level in conidiophores and vegetative hyphae during asexual development, and strongly expressed in the shells of young cleisthothecia and Hulle cells at the early sexual stage. The expression of the chitin synthase genes, chsA, chsC, and chsD, except for chsB, was altogether enhanced by high concentrations of salts, such as KCl and NaCl.

abstract No: 

Fungal Genet. Newsl. 50 (Supl):abstract

Full conference title: 

22nd Fungal Genetics Conference
    • Fungal Genetics Conference 22nd (2001)