As one of the consecutive efforts to understand the role of the chitin synthase genes of Aspergillus nidulans, we analyzed the mode of expression of the chitin synthase gene, chsE, both by Northern blotting and by a vital reporter system with sgfpencoding a modified version of green fluorescent protein (sGFP). On a minimal medium containing 1% glucose as a carbon source, chsE was expressed at a considerable level in conidiophores and at a somewhat lower level in vegetative mycelia. During sexual development, chsE was strongly and specifically expressed in young cleistothecia and Hulle cells at early stage, however, was not expressed either in mature cleistothecial shells, in ascospores, or in Hulle cells. The level of chsE expression was increased to some extent by substituting sucrose or lactose for glucose contained in the medium as a sole carbon source, however, was slightly reduced by sodium acetate. While osmostress caused by high concentrations (up to 1.2 M) of KCl, NaCl, or sorbitol stimulated the expression of chsE, heat shock did not affect its expression at all. Consequently, it is suggested that chsE has quite a distinctive mode of expression and function from those of the other chitin synthase genes of this fungus, such as chsA, chsB, and chsC.
Full conference title:
23rd Fungal Genetics Conference
- Fungal Genetics Conference 23rd (2002)