Different roles of the mre11 complex in the DNA damage response in Aspergillus nidulans.

Gustavo H. Goldman, Camile P. Semighini, Márcia R. V.Z. Kress Fagundes, Joseane C. Ferreira, Renata C. Pascon, Maria Helena S. Goldman

Abstract: 

The hMRE11-hRAD50-NBS1 protein complex has emerged as a central player in the cellular DNA damage response. Mutations in scaANBS1, which encodes the apparent homolog of human nibrin in A. nidulans, inhibit growth in the presence of anti-topoisomerase I drug camptothecin. We have used the scaANBS1cDNA as a bait in a yeast two-hybrid screening and report the identification of the A. nidulans Anmre11 homologue. The inactivated Anmre11 strain (TMRE11) was more sensitive to several DNA damaging and oxidative stressing agents. Septation in A. nidulans is dependent not only on the uvsBATR gene but also on the mre11 complex. scaANBS1 and Anmre11 genes are both involved in DNA replication checkpoint while Anmre11 gene is involved in the intra-S-phase checkpoint. SCAANBS1 and ANMRE11 also participate in G2-M checkpoint upon DNA damage caused by 4-NQO. Homologous recombination is affected by the scaA1 mutation since this mutant does not have the ability of integrating exogenous DNA introduced by DNA-mediated transformation in a homologous way. The scaANBS1 gene is important for ascospore viability while Anmre11 gene is essential for the sexual fertility in A. nidulans. In addition, the mre11 complex and uvsCRAD51 genes are highly expressed at mRNA level during the sexual development. Financial support: CNPq and FAPESP, Brazil
2003

abstract No: 

Fungal Genet. Newsl. 50 (Supl):abstract

Full conference title: 

22nd Fungal Genetics Conference
    • Fungal Genetics Conference 22nd (2001)