Diagnosis of Invasive Pulmonary Aspergillosis (IPA) Using Polymerase Chain Reaction (PCR) Based Detection of Aspergillus in Brochoalveolar Lavage (BAL)

ISSAM I RAAD, ARMANDO HUARINGA, DAVID SUMOZA, HEND A HANNA, KENNETH V ROLSTON, DIMITRIOS P KONTOYIANNIS, RAY Y HACHEM, end MAHER ALBITAR

Abstract: 

Background: IPA is associated with high morbidity and mortality in cancer patients and is difficult to diagnose. Most of the polymerase chain reaction (PCR) studies on BAL were retrospective with a small number of high risk patients. Methods: Between January 1996 and March 1997 we prospectively followed 249 cancer patients at risk for IPA. Bronchoscopy with fungal steins, cultures and PCR was performed on all patients. PCR was used for the detection of Aspergillus mitochondrial (Min) and alkaline wee (AP) gene DNA. The PCR products were either visualized directly on polyacrylamide gel or after Southern transfer and probing with specific probes for Mit and AP DNA. Results: The 249 patients consisted of 10 with definite IPA (tissue invasion), 22 with probable IPA (microbiologic culture). 1 S with possible IPA (consistent clinical and radiologic findings). and 199 control patients with no evidence of IPA. PCR positivity was strongly associated with all forms of IPA (P
2000

abstract No: 

NULL

Full conference title: 

IDSA 38th
    • IDSA 38th