Diagnosing fungal infections by mass spectrometry

J. Nedved, M. Kuzma, M. Strohalm, K. Lemr, M. Sulc, M. Volny, P. Novak, J. Pol, V. Havlicek, E. Barreto- Bergter, M. Hajduch, J.M. Holopainen

Author address: 

Prague, CZ; Rio de Janeiro, BR; Olomouc, CZ; Helsinki, FI

Abstract: 

Objectives and Method: Mass spectrometry is presented as a modern analytical tool for fungal strain typing and diagnosing fungal infections. Results: Qualitative and quantitative proteomics approaches are documented on various Aspergillus strains: virulence protein factors present on fungal spores are identified by peptide mapping, peptide- and de novo-sequencing. Quantitative proteomics is addressed by NOVA-Q in house-developed software improving the precision of results in samples labeled by SILAC. Metabonomics approach is illustrated by the detection of minor macrolide antibiotics produced by Streptomyces strains. Peptidomics is represented by tracking non-ribosomal cyclic peptides and depsipeptides produced by Beauveria, Paecilomyces and Pseudallescheria genera. Peptide profiles are used as chemotaxonomic tools. Patented unique nonribosomal lasso-peptide structures are reported as extremely specific fungal markers. Glycomics and lipidomics armory is illustrated by hexosylceramide analysis in Scedosporium by Fourier-transform ion cyclotron resonance mass spectrometry. Clinical samples (tissues, whole blood) handling by advanced ambient ionization techniques is reported with special focus to lipids in brain, eye bulb and lungs (murine, porcine). The fungal infections in plants is addressed by DAPPI mass imaging. Conclusion: The current advances in mass spectrometry will lay the experimental foundation for modern sensitive diagnostic tools. We predict that particularly mass imaging of tissues infected by molds will lead to discovery of specific fungal biomarkers.
2010

abstract No: 

O540

Full conference title: 

20th European Congress of Clinical Microbiology and Infectious Diseases
    • ECCMID 20th (2010)