DIAGNOSING AND MONITORING OF INVASIVE ASPERGILLOSIS DURING ANTIFUNGAL THERAPY BY POLYMERASE CHAIN REACTION: AN EXPERIMENTAL STUDY IN MICE

Lass-Florl C, Speth C, Mayr A, Wurzner R, Dietrich H

Author address: 

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Abstract: 

Objectives: Invasive aspergillosis is one of the commonest causes of death due to infection in neutropenic cancer patients and recipients of allogenic stem cell transplants. Early diagnosis of infection is important as early treatment with antifungal drugs may increase the patient survival. Recently, we evaluated the value of twice weekly screening for circulating fungal DNA using polymerase chain reaction (PCR) of whole blood samples. The results indicate the usefullness when screening for Aspergillus in patients at risk. Hence, positive results became negative shortly after commencment of antifungal treatment and did not correlate with underlying invasive fungal infections. In this study we evaluated the value of PCR for diagnosing and monitoring of invasive aspergillosis during amphotericin B therapy. Methods: 36 female BALB/c mice were infected with Aspergillus spp. Mice were treated either with amphotericin B or glucose for 10 days. For diagnosing and monitoring PCR, microscopy and culture of tissues and blood were performed. The PCR results of treated were compared to those of untreated animals. DNA extraction was performed using recombinat lyticase, the genes of the 18S rRNA were amplified with PCR using specific primers and amplicons were detected by PCR-ELISA. Results: In the group of amphotericin B application 27 (64.3%) tissue samples were positive by PCR, 32 (76.1%) by microscopic examination, and 20 (47.6%) by culture. In the control group 30 (71.4%) tissue samples were positive by PCR, 31 (73.8%) by microscopic examination, and 28 (66.6%) by culture (p = 0.34). Of the 48 blood samples tested over a period of 8 days following Aspergillus infection, 14 (58.3%) samples in the control group and 7 (29.1%) in the amphotericin B group were positive (p
2003

abstract No: 

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Full conference title: 

The 15 th Congress of the International Society for Human and Animal Mycology
    • ISHAM 15th (2003)