Development of URP-PCR primers for screening genetic diversity of fungal species

A Min Kwak, Jong Kun Kim, Hee Wan Kang

Author address: 

Graduate School of Bio.&Information Technology, Hankyong National University, Ansung 456-749, Korea

Abstract: 

URP primers were developed from DNA repetitive sequence of Korean weedy rice. URP-PCR protocol employed stringent PCR with high annealing temperature over 558451; throughout the thermo-cycling reaction, giving high reproducibility. Under the PCR condition, each single URP primer produced characteristic fingerprints from diverse genomes containing plants, animals and microbes, indicating its universal applicability. URP-PCR technology has been applied for accessing genetic diversity of various fungal species with 33 genus, 142 species and 1,489 isolates. Numerous papers using URP-PCR have demonstrated that URP-PCR profiles of fungal species are very useful for identifying fungal species at intra and inter species levels and further the specific PCR polymorphic bands could be used for SCAR PCR primer for diagnosis of target species or strains.
2013

abstract No: 

P-36

Full conference title: 

Asian Mycological Congress 2013 and the 13th International Marine and Freshwater Mycology Symposium
    • AMC 2013