Development of a novel inducible expression system for the production of heterologous proteins in Aspergillus: successful production of lignolytic enzymes

Sandra de Weert1, Nick van Biezen2, Dennis Lamers2, Jeroen Schouwenberg2, Kees van den Hondel1,2, Arthur Ram1 and Christien Lokman1,

Author address: 

1 Institute of Biology Leiden, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands

Abstract: 

For industrial applications and (medical) scientific research efficient (heterologous) production of compounds like, enzymes, antibodies, viral epitopes, chemical compounds and antibiotics is of great importance. For these purposes Aspergillus functions as a suitable production host. Over the years all kinds of commercially available expression systems have been developed. A well established expression system is the one based on the protein Glucoamylase (GlaA). However, in the case of heterologous protein production the efficiency of these systems is still very depending on the protein to be produced. Recently, we identified the inuE gene, encoding for the exo-inulinase protein in A. niger, as the most strongly induced gene in the presence of inulin and sucrose (Yuan et al., 2006; Yuan et al., 2007). Characteristics of the system were studied by placing gfp behind the inuE promoter. This reporter strain showed that the inuE gene is highly expressed when grown on inulin and sucrose. No expression was observed when grown on glucose, fructose and xylose indicating a tight control on different carbon sources. This tight control can be a benefit if the heterologous protein to be produced can be a disadvantage for fungal growth. Finally, different peroxidases and a laccase were successfully produced in Aspergillus using this novel inducible expression system.
2010

abstract No: 

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Full conference title: 

10th EUROPEAN CONFERENCE ON FUNGAL GENETICS
    • ECFG 10th (2010)