Development of molecular markers for studying the genetic basis of natural phenotypic variation in Aspergillus nidulans

Julian Christians1, Cortney Watt2

Author address: 

1Simon Fraser University, Burnaby, BC, Canada, 2St. Francis Xavier University, Antigonish, NS, Canada

Abstract: 

Introduction: Studying natural phenotypic variation within species will contribute to our understanding of the genes underlying traits of medical, industrial and evolutionary importance. Approaches for studying such variation, including quantitative trait loci (QTL) mapping, require many molecular markers. Microsatellites are a commonly-used marker in other organisms, but are relatively difficult to develop in fungi. Methods: This study examined the relative polymorphism rates of mononucleotide, dinucleotide and trinucleotide repeats among four wild-type strains of Aspergillus nidulans. Results: The probability of polymorphism increased with number of repeating units. Di- and trinucleotide repeats had higher polymorphism rates than mononucleotide repeats, but this was offset by the presence of numerous long mononucleotide repeats. Discussion: The use of mononucleotide repeats will substantially increase the number of potential markers available for studies of Aspergillus nidulans. Given that mononucleotide repeats are more abundant than other repeats in fungal genomes in general, mononucleotide repeats are likely to be an important resource in other species as well.
2008

abstract No: 

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Full conference title: 

9th EUROPEAN CONFERENCE ON FUNGAL GENETICS
    • ECFG 9th (2008)