DEVELOPMENT OF AN INHALATIONAL MODEL OF PULMONARY ASPERGILLOSIS IN CHICKENS

S. Thierry1*, J. Tafani2, M. Deville1, R. Chermette1, J. Guillot1, P. Arné1

Author address: 

1UMR AFSSA, ENVA, UPVM BIPAR, Ecopham, Ecole Nationale Vétérinaire d’Alfort, Maisons-Alfort, France 2APcis, Maisons-Alfort, France

Abstract: 

Purpose: Aspergillosis has been described worldwide in a very large number of avian species. Turkey poults in large confinement houses, quails, marine birds that are brought into rehabilitation, captive raptors, and penguins being maintained in zoological parks commonly die from aspergillosis. Aspergillosis occurs in immunocompromised birds or in birds exposed to overwhelming numbers of conidia. In most cases, the primary site of development is the respiratory tract (air sacs and lungs) but blood dissemination frequently occurs leading to macroscopic lesions in a wide range of organs or tissues. The use of experimental models is required to better understand host-pathogen interactions and develop diagnostic and therapeutic tools. Experimental aspergillosis has already been described in various domestic species, especially chickens and turkeys. Methods: The aim of the present study was to develop an inhalational model for birds. Five-day-old chicks (SPF Leghorn PA12) were systematically used. Results: The effect of several parameters was evaluated (i) the concentration of Aspergillus fumigatus conidia in air, (ii) the duration of inhalation, (iii) the single or repetitive inoculation at 48h interval and (iv) the immune status of the birds. All the animals were weighed and examined daily from 1 to 7 days post inhalation. The animals were sacrified. Their lungs were harvested and homogenized with sterile saline. Conclusions: Fungal development was assessed by culture. Histopathological examination was also performed.
2010

abstract No: 

170

Full conference title: 

4th Advances Against Aspergillosis
    • AAA 4th (2010)