Aspergillus fumigatus is an opportunistic fungal pathogen of man causing a wide spectrum of diseases some of which are fatal. To facilitate the discovery of efficient anti-fungal drugs we have identified genes that are required for fungal growth in vitro. To verify the potential of putative targets we have developed a conditional gene expression system in A. fumigatus. An important consideration for development was the ability of the expression system to act in any host models of fungal infection. We have previously demonstrated the use of wax moth larvae Galleria mellonella as a model for gene expression studies during A. fumigatus infection. The development of expression systems that can be manipulated in this and a more standard mouse model host are discussed in this study. The expression of a selection of A. fumigatus genes has been assessed in vitro. Two genes were identified (cbaA and cbhB) which were strongly expressed in the presence of an inducer (CMC) but repressed in the presence of glucose. Expression of cbaA and cbhB was not detected during infection of G. mellonella. Data will be presented on the expression of these genes during infection in the presence of the inducer. The promoter regions of these genes have been used as the basis for construction of expression cassettes in A. fumigatus. Data will be presented on the expression of a reporter gene in the cassette.
Fungal Genet. Newsl. 50 (Supl):abstract
Full conference title:
22nd Fungal Genetics Conference
- Fungal Genetics Conference 22nd (2001)