Determination of Plasmid Copy Number in Yeast

Autonomously replicative plasmids are widely employed as vectors for the expression of cloned genes in yeast (1). A number of different kinds of such plasmid vectors exist, which fall into three classes: ARS (autonomously replicating sequence) based plasmids, 2-µm-based plasmids, and ARS plasmids containing a centromere.

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