Introduction / objectives: Outbreaks of nosocomial mucormycosis have been reported to environmental sources, usually consist of medical devices in direct contact with patients. Apart from these episodes, Mucorales are rarely reported in the hospital environment (air, water, surfaces). Following the investigation of three cases grouped based on the contamination of resuscitation equipment, we wanted to evaluate fungal contamination of devices in the Intensive Care unit of our hospital. Methods: The swabs were made on the back electrical appliances present in the wards and reserves of 3 service Resuscitation. The swabs were passed over the fins and the cooling fans of the gates of devices, and seeded on Sabouraud liquid and solid for 7 days at 30 ° c. Membership in order Mucoraleswas conducted by macroscopic observation of colonies and microscopic observations of filaments and spores. The results were made in qualitative (presence / absence). Results: Between 07/01 and 01/03/2010, 72 biomedical devices were sampled. Strains of Mucorales were isolated from 13 of 40 respirators (32.5%), 2 of 6 dialysis machines, computers 2 7, 1 of 2 mattresses engines and personal fan. In addition, strains of Aspergillus sp. were found on 30% of respirators, 29% of computers 1 and 17 scopes. At the same time, the common control of bio contamination of air and surfaces showed no significant fungal contamination in these services. Discussion / Conclusion: The filamentous fungi have a propensity to adhere to hot surfaces that have electrostatic grids and cooling fins of bio medical equipment, including respirators, present in the resuscitation services. These small areas difficult to clean routine maintenance (need to disassemble the unit), are an environmental reservoir not described in the literature and not detected when measuring aerobiocontamination. The clinical significance of this endemic contamination remains to be assessed by monitoring of mucormycosis and immediate environmental investigations around the possible cases.
Full conference title:
- RICAI 30th (2010)