Trichophyton tonsurans is distributed in Europe, America, Australia and Southeast Asia but few isolate has been reported in Japan. Recent a couple of years the outbreak of tinea capitis caused by T. tonsurans have been reported all over the Japan. Almost all patient were judo or wrestring players.Because the clinical features of the T. tonsurans infection are mild and not easy to diagnose, quick and reliable means of detection and identification are desired to control the dermatophytosis. To solve this problem, we developed non-culture based detection and identification method for T. tonsuran from clinical and environmental materials using specific PCR. The T. tonsurans specific primer-pair was designed in the DNA sequences of nuclear ribosomal Internal Transcribed Spacer 1. The specificity of the primer were tested in major dermatophytes and environment of fungi. All of 6 T.tonsurans were positive by PCR with the specific primer pair, however other fungi including, 29 stains of T. mentagrophytes, 44 stains of T. rubrum T. schoenleini, T. verrucosm, T. violaceum, Arthroderma vanburuseghemii, Micorsporum gypseum, M. canis, Epidermophyton floccosum, Aspergillus nudurans, A. japanicus, A. terreuse, A. versicolor, Malassezia furfur, M. sympodialis, were negatine. Using PCR with the primer-pair we could detect directly T. tonsurans from clinical specimen and environmental samples including cloth. The presented T. tonsurans specific PCR method will be a powerful tool for early diagnosis, treatment, and preventing the outbreak caused by the pathogen.
Full conference title:
The 15 th Congress of the International Society for Human and Animal Mycology
- ISHAM 15th (2003)