Blaster cassettes are of significant value in functional genomics, as they represent tools to inactivate duplicated or homologous genes in an individual organism subsequently. We have constructed a novel blaster module for the potential use of repeated gene deletion in the filamentous fungus Aspergillus nidulans. Due to the employment of bacterial resistance marker cassettes as direct flanking repeats, the blaster cassette is suited for recombinogenic engineering by ET cloning in E. coli. Functionality of the blaster module was demonstrated by deleting the chorismate mutase-encoding gene aroC of A. nidulans followed by marker rescue based on mitotic recombination. The resulting aroC Delta strains are auxotroph for phenylalanine but not tyrosine and display limited capacities of fruit body formation and ascosporogenesis, dependent on the phenylalanine/tyrosine supply. The data support the strong impact of the amino acid status on cleistothecia development in A. nidulans.
Fungal Genet. Newsl. 50 (Supl):abstract
Full conference title:
22nd Fungal Genetics Conference
- Fungal Genetics Conference 22nd (2001)