Cross Reactivity in the Platelia Aspergillus Galactomannan Antigenemia Assay Caused by Histoplasmosis.

L. J. WHEAT1, E. HACKETT 1, M. DURKIN 1, T. WALSH 2, K. KNOX 3, C. HAGE 4, C. HAGE 4;

Author address: 

1Mira Vista Diagnostics, Indianapolis, IN, 2Natl. Institutues of Health, Washington, DC, DC, 3Indiana Universtiy Sch. of Med., Indianapolis, IN, 4Indiana Univ. Sch. of Med., Indianapolis, IN.

Abstract: 

Background. We have recently observed positive results in the Platelia Aspergillus galactomannan antigenemia assay in serum specimens from four patients with histoplasmosis. The purpose of this study was to evaluate the frequency of such cross-reactivity. Methods. Clinical and animal model specimens containing high levels of Aspergillus galactomannan or Histoplasma antigen were tested in the Aspergillus galactomannan assay and Histoplasma antigen assay. Purified Histoplasma and Aspergillus galactomannan also were studied for cross-reactivity. Results. Twenty-three of 48 serum (48%) and 7 of 11 (64%) bronchoalveolar lavage fluid (BAL) clincal specimens containing Histoplasma antigen were positive in the Aspergillus galactomannan assay. Twenty serum and 18 BAL clinical specimens containing high levels of Aspergillus galactomannan were negative in Histoplasma antigen assay, however. Similarly, spleen from three of 11 (27%) B6C3F1/J mice with histoplasmosis were positive in the Aspergillus galactomannan assay, while serum and BAL from eight rabbits with aspergillosis were negative in Histoplasma antigen assay. Histoplasma galactomannan was detected at
2006

abstract No: 

M-366a

Full conference title: 

46th Interscience Conference on Antimicrobial Agents and Chemotherapy
    • ICAAC 46th