Correlation of Serum Precipitins and IgG Anti Aspergillus Fumigatus (IgG-Af) Determined by Fluoroenzymeimmunoassa

P. S. Hutcheson, R. G. Slavin

Author address: 

Internal Medicine, Saint Louis University School of Medicine, St. Louis, MO,

Abstract: 

RATIONALE: The presence of serum precipitins against Aspergillus fumigatus (Af) and IgG-Af are considered part of the diagnostic criteria for allergic bronchopulmonary aspergillosis (ABPA). There is no data available on the specific lgG UniCAP assay, so we compared serum precipitins to Af and IgG-Af. METHODS: Precipitin determinations consisted of sera placed in gel surrounded by 5 different antigen Af preparations and were scored 0-5 depending on how many antigens were positive. IgG-Af was determined by UniCAP 100 fluoroenzymeimmunoassay (Pharmacia) with results in mg/l. The determinations were done on 161 patient samples: (group 1) 35 CF Af sensitive (skin test +) non-ABPA; (group 2) 47 CF Af non-sensitive nonABPA; (group 3) 40 asthmatic ABPA; (group 4) 37 CF ABPA. RESULTS: The majority of precipitin scores were either 0 or 5, and there was a trend toward higher IgG-Af with increased numbers of precipitins, but this was not statistically significant. The mean for each of the four groups with 0 and 5 precipitin scores respectively was: group 1-32.8, 125.9; group 2-25.6, 122.1; group 3-64.8, 122.9; and group 4-53.4, 129.0. The range in nonABPA patients with 0 precipitins never exceeded 105, whereas the range in ABPA patients with 5 precipitins never fell below 30. CONCLUSION: The presence of precipitins did not significantly correlate with increased IgG-Af, although there was a trend toward higher IgGAf associated with higher precipitin scores. It would appear that both serum precipitins and IgG-Af determinations should be done as part of the serology to establish the diagnosis of ABPA. Funding: NIH and CF Foundation
2003

abstract No: 

S294

Full conference title: 

2003 American Academy of Allergy, Asthma, and Immunology Annual Meeting
    • AAAAI 2003 (59th)