Concomittant N-Acetylcystein for the Prevention of the Amphotericin B Induced Renal Tubular Apoptosis.

Z. ODABASI1, A. KARAALP 1, H. CERMIK 2, J. MOHR 3, O. ERGONUL 1, Z. AGALAR 1, B. SUMER 1, L. MULAZIMOGLU 1, V. KORTEN 1;

Author address: 

1Marmara Univ., Istanbul, Turkey, 2Etimesgut Military Hosp., Ankara, Turkey, 3Univ. of Texas Houston Med. Sch., Houston, TX.

Abstract: 

Background: Nephrotoxicity is one of the serious and dose limitting side effects of amphotericin B (AmB). AmB induced nephrotoxicity is caused by renal vasoconstriction and renal tubular dysfunction secondary to apoptosis of renal tubular cells. N-acetylcystein (NAC) is an anti-apoptotic drug and it is currently used for the prevention of radiocontrast induced nephrotoxicity. We measured the effect of NAC on the degree of AmB induced renal tubular cell apoptosis. Methods: Four groups of Sprague-Dawley rats weighing avarage 200g (10 in each group) were treated with 10 mg/kg/day intraperitoneal (IP) AmB for 5 days. Each group was also given IP sterile water (group A), IP NAC 30 mg/kg bid (group B), or 60 mg/kg bid (group C) or 120 mg/kg bid (group D). Kidney tissue sections were examined for apoptosis by the terminal deoxynucleotidyl transferase mediated dUTP nick end-labeling (TUNEL) technique. For estimation of the level of apoptosis, the TUNEL-positive renal tubular epithelial cells as well as unstained cells were scored in 10 different fields at x400 magnification and the apoptotic index (AI) was expressed as the percentage of total cells scored. Results: Results are summarized in the table below. Average weights of animals in group A (159g) was lower than groups B (205g), C (207g) and D (211g). Animals recieving NAC had a reduction in apoptosis compared to animals receiving AmB alone (p
2006

abstract No: 

A-1100

Full conference title: 

46th Interscience Conference on Antimicrobial Agents and Chemotherapy
    • ICAAC 46th