Complementation of the Aspergillus nidulans wetA mutant with the apparent Neurospora crassa ortholog NCU01033

Srijana Upadhyay, Daniel J. Ebbole, Heather H. W ilkinson, and Brian D. Shaw

Author address: 

Department of Plant Pathology and Microbiology, Program for the Biology of Filamentous Fungi, Texas A&M University, College Station TX 77843

Abstract: 

The asexual life cycle of Aspergillus nidulans begins and ends with the production of uninucleate haploid spores called conidia. A. nidulans genes brlA, abaA and wetA are considered key regulators of conidiophore development and conidium maturation. In A. nidulans wetA is required for synthesis of cell wall layers that make spores impermeable. The wetA mutant of A. nidulans displays defective conidia that fail to mature and later undergo lysis. The Neurospora crassa deletion mutant of the wetA ortholog (NCU01033) does not show any defect in conidiation. This study assesses the ability of these genes to cross complement. A chimeric fusion construct with the A. nidulans wetA promoter and the N. crassa NCU01033 coding sequence restored the conidiation defect of the A. nidulans wetA mutant. Complementation analysis of wetA mutant with N. crassa NCU01033 expressed by its endogenous promoter is ongoing.
2009

abstract No: 

16

Full conference title: 

6th International Aspergillus Meeting
    • Asperfest 6 (2009)