Comparison of the Vitek AST-YS01 card with CLSI broth microdilution reference method for testing fluconazole and voriconazole against C. neoformans

Aller A.I., Castro C., Romero A., González M.T., Claro R., Martí­n-Mazuelos

Author address: 



Objective: The purpose of this study was to compare the results obtained by the reference microdilution method (MDM) with those obtained with the comercial method AST-YS01card (YS01) for antifungal susceptibility testing of C. neoformans to fluconazole (FZ) and voriconazole (VZ) Materials and methods: We have studied retrospectively 89 clinical isolates of C. neoformans. The susceptibility to two azoles was performed by the MDM according to the CLSI guidelines (M27-A2 document) modified by Ghannoum et al (J. Clin. Microbiol. 1992; 30:2881-86). The MICs were read at 48-72 h. of incubation. The YS01 was performed according to the manufacturer’s instructions. The inoculum suspensions of C. neoformans isolates matched the turbidity of nº2 McFarland standard. The MICs were read at 24-48 h. of incubation. The interpretative criteria for VZ were those published by the CLSI (Document M27-A3 and M27-S3). For FZ the isolates with MIC>=16 mg /L were considered resistant and the isolates with MIC

abstract No: 

P 1307

Full conference title: 

19th European Congress of Clinical Microbiology and Infectious Diseases
    • ECCMID 19th (2009)