Comparison of biofilm formation ability among Candida clinical isolates.

A. Silva-Dias, I. Miranda, C. Pina-Vaz, A.G. Rodrigues

Author address: 

University of Porto, PORTO, Portugal

Abstract: 

Objectives: C. albicans is the Candida species most associated with mucocutaneous and systemic mycosis, but recently, non-albicans species have been increasingly responsibility for such conditions. The ability of Candida species to form biofilms has important clinical repercussions since it results in a reservoir of cells with promoted antifungal resistance. Our aim was to evaluate the biofilm formation by distinct Candida clinical strains (isolated from patients admitted at Hospital S. Joí£o) regarding their metabolic activity and total biomass. Methods: Clinical isolates of Candida (n=165), corresponding to C. albicans (n=45), C. parapsilosis (n=45), C. glabrata (n=45) and C. tropicalis (n=30), were obtained from different human samples. Biofilm were quantified colorimetrically with a crystal violet assay and a XTT assay after 24 and 48 hours of incubation. Results: Data obtained using the above methodologies were discrepant: a lack of correspondence between biomass formation and metabolic activity was found. C. tropicalis showed the higher ability to form biofilm followed by C. parapsilosis , C. glabrata and C. albicans when quantified by crystal violet. However quantification with XTT assay showed that, C. albicans strains showed higher biofilm metabolic activity followed by C.glabrata , C. parapsilosis and C. tropicalis .A marked variability intra and inter species was noticed. Conclusion: C. albicans despite being the species with higher metabolic activity displayed lower biomass formation comparing to non-albicans species. For all species, biofilm formation showed to be strain or species dependent.
2009

abstract No: 

P261

Full conference title: 

4th Trends in Medical Mycology
    • TIMM 4th (2012)