COMPARATIVE STUDY OF ANTIFUNGAL ACTIVITIES OF HUMAN NEUTROPHILS AGAINST THE ZYGOMYCETES RHIZOPUS ORYZAE, RHIZOPUS MICROSPORUM AND ABSIDIA CORYMBIFERA

Roilides E 1 , Simitsopoulou M 1 , Gil-Lamaignere C 1 , Maloukou A 1 , Winn RM 1 , Walsh TJ 2

Author address: 

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Abstract: 

Background: Zygomycetes cause infections with high morbidity and mortality in diabetic and immunocompromised patients. Neutrophils (PMNs) are a critical component of antifungal host defense. The antifungal activities of PMNs, specifically the oxidative burst and hyphal damage, against R. oryzae (RO), R. microsporum (RM) and A. corymbifera (AC) were compared. Methods: PMNs were isolated from healthy donors by dextran sedimentation/ ficoll centrifugation. The oxidative burst, evidenced by superoxide anion (O2 - ) released by PMNs, in response to hyphae of RO, RM and AC at effector: target cell ratio 1:1 at 37 o C for 1 h was determined by cytochrome c reduction assay. The PMN-induced % hyphal damage was assessed by colorimetric XTT assay after incubation of PMNs with hyphae at ratios 5:1, 10:1 or 20:1 for 1 h. Statistical analysis of 7-8 experiments was performed by ANOVA, with Bonferroni post-test. Results: Serum opsonized (op) and non-opsonized (nop) hyphae of all three species induced higher PMN O2 - production (2.5+0.5 nM O2 - for nop RO and 3.2+0.6 for op RO; 2.6+/-0.4 for nop RM and 3.1+/-0.4 for op RM; 3.0+/-0.5 for nop AC and 3.6+/-0.5 for op AC) than unchallenged PMNs (1.2+/-0.2; p
2003

abstract No: 

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Full conference title: 

The 15 th Congress of the International Society for Human and Animal Mycology
    • ISHAM 15th (2003)