Using the high resolution RNA8208;Seq, we have analyzed the different mRNA expression patterns between vegetative and asexually developing mycelia of Aspergillus nidulans. The pathways and genes that are activated and repressed during vegetative hyphal growth and conidiophore production have been identified. We also describe a substantial number of novel transcripts that are controlled by Upstream Developmental Activators (UDAs, especially FlbB) at different stages of development. Genes involved in secondary metabolism (like polyketide synthases, see poster Rodríguez8208;Urra et al.), increased oxidoreductase activity and/or transcription factors (principally binuclear zinc clusters) are examples of groups regulated by FlbB. Our analysis indicates that during asexual development the 4% of the transcriptome is modified comparing with vegetative growth, including more genes than previously were anticipated. Of these, FlbB regulates the 60% directly or indirectly. These results may provide a blueprint for further study of the Aspergillus nidulans development.
Full conference title:
11 th European Conference on Fungal Genetics
- ECFG 11th (2012)