Comparative Functional Pathway Analysis of the Genomic Profiles of Human Monocytes in Response to Aspergillus fumigatus and Rhizopus oryzae.

K. J. CORTEZ1, C. A. LYMAN 1, R. LEMPICKI 2, P. REN 2, J. YANG 2, C. J. COTTEN 1, E. ROILIDES 1, S. KOTTILIL 3, D. KONTOYIANNIS 4, T. J. WALSH 1;

Author address: 

1NCI, NIH, Bethesda, MD, 2SAIC, Frederick, MD, 3NIAID, NIH, Bethesda, MD, 4MDACC, Houston, TX.

Abstract: 

Background: Previously we have reported on the comparative gene expression profile of innate immunity molecules induced on monocytes in response to the emerging pathogens Aspergillus fumigatus (AF) and Rhizopus oryzae (RO). In an attempt to understand the significance of the differential genomic profile we investigated the gene expression in various pathways of innate immunity. Methods: MNCs (1x107cells/ml) from 10 normal volunteers were incubated with and without freshly harvested AF conidia and RO sporangiospores (E: T ratio 10:1) for 0, 2, 4 and 6 h. cRNA was hybridized onto Affymetrix® Gene Chips HG133 Plus 2.0. Mixed model ANOVA was performed. Functional annotation and pathways analysis were performed using DAVID (version 2, NAID). Genes with p-values
2007

abstract No: 

M-1837

Full conference title: 

47th Interscience Conference on Antimicrobial agents and Chemotherapy
    • ICAAC 47th