Comparative Functional Genomics of Innate Host Defense Molecules in Human Monocytes Infected with Aspergillus fumigatus and Rhizopus Oryzae.

K. J. CORTEZ1, C. A. LYMAN 1, R. LEMPICKI 2, P. REN 2, J. YANG 2, C. COTTEN 1, E. ROILIDES 1, D. P. KONTOYIANNIS 3, S. KOTTILIL 2, T. J. WALSH 1;

Author address: 

1NCI, Bethesda, MD, 2NIAID, Bethesda, MD, 3MDACC, Houston, TX.

Abstract: 

Background: Little is known about comparative functional genomic expression associated with innate immune responses of human phagocytic cells to pathogenic fungi. We therefore investigated and compared the kinetics of early gene expression of innate host defense molecules in human monocytes (HMCs) infected with A. fumigatus and R. oryzae by DNA microarray analysis. Methods: Elutriated HMCs (1x107cells/ml) from 10 normal volunteers were incubated with and without freshly harvested A. fumigatus conidia and R. oryzae sporangiospores (E:T ratio 10:1) for 0, 2, 4 and 6 h. Total RNA was extracted with Trizol, amplified, hybridized onto Affymetrix Gene Chips HG133 Plus 2.0, and analyzed with an Affymetrix Scanner. Mixed model ANOVA was performed with donors set as a random effect, and time and treatment as main effect. Genes with p-values
2006

abstract No: 

M-1773

Full conference title: 

46th Interscience Conference on Antimicrobial Agents and Chemotherapy
    • ICAAC 46th