Comparative evaluation of PCR and serology for diagnosis of aspergillosis in patients with hematologic malignancies

J. Poth, E. Engelmann, M. Mielke, D. Huhn, M. Puhnke.


Objectives: In this study we compared the diagnostic value of two different methods that both promise to provide early and sensitive detection of invasive aspergillosis (IA).Methods: We used a PCP,-based method which amplifies a fungus-specific 18S RPNA fragment with subsequent species-specific hybridization (according to Einsele et al; DNA extraction had been modified) as well as the Platelia Aspergillus antigen detection system (Sanofi Pasteur). Within a period of 18 months, blood and BAL (if available) of 34 patients with hematologic malignancies (NHL, n=7; AML, n=18; ALL, n=3; CML, n=6) were prospectively sampled once or twice a week during hospital stay and processed as described above (samples: Y=226 ). Sixteen patients showed no clinical or laboratory evidence for IA (group A), and 12 patients had clinical and radiologic signs of probable IA (group B). Group C was composed of six patients with proven IA (five histologically proven, one positive in repeated BAL).Conclusion: The Platelia test appeared to provide more conclusive results in proven IA than the PCR-based method.

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9th European Congress of Clinical Microbiology and Infectious Diseases
    • ECCMID 9th (1999)