Comparative Analysis of Etest and Broth Microdilution Method (AFST- EUCAST) for Trends in Antifungal Drug Susceptibility Testing of Brazilian Cryptococcus neoformans isolates

Dias1,2, A.L.T., Matsumoto3, F.E., Melhem4, M.S.C., Da Silva3, E.G., Auler3, M.E., Siqueira2, A.M., Paula3, C.R.

Author address: 

Alfenas- MG DCB,ALFENAS, Brazil 2Unifal-MG, DCB, ALFENAS, MG, Brazil 3USP, Laboratório de Micologia, SíƒO PAULO, Brazil 4IAL, SíƒO PAULO, Brazil


A prospective study was performed to evaluate the correlation among the proposed standard of the Antifungal Susceptibility Testing Subcommittee of the European Committee on Antibiotic Susceptibility Testing (AFST-EUCAST) (document 7.1) and the commercial system Etest for determining the Minimum Inhibitory Concentrations (MICs) of flucytosine, amphotericin B, fluconazole, itraconazole and voriconazole for a collection of 100 clinical and environmental isolates of Cryptococuus Neoformans. The agreements among Etest MICs within +-2 log2 dilutions of AFST-EUCAST standard MICs were higher to flucytosine, fluconazole and voriconazole (76 %, 78 % and 88 %, respectively) than to the lowest agreement of amphotericin B (5 %) and itraconazole (67 %). Overall, the correlation coefficients were statistically significant (P

abstract No: 


Full conference title: 

3rd Trends in Medical Mycology
    • TIMM 3rd (2011)