Cloning of the GCN4/cpc-1 Homologue of Aspergillus niger

Sabine Eckert, Christoph Wanke, Wim v. Hartingsfeldt, Peter Punt, Cees van den Hondel and Gerhard Braus.

Author address: 

Friedrich-Alexander-University, Institute for Biochemistry, Microbiology and Genetics, Staudtstr. 5, 91058 Erlangen


TNO Nutrition and Food Research Institute, Molecular Genetics and Gene Technology, P.O.Box 5815, 2280 HV Rijswijk, The Netherlands Imbalances in the pool of amino acids in certain fungi is known to result in a coordinated derepression of amino acid biosynthetic genes. This phenomenon, called General Control of amino acid biosynthesis in Saccharomyces cerevisiae (Hinnebusch, 1992) and Cross-Pathway control in Neurospora crassa (Carsiotis et al., 1974), was also known to exist in Aspergillus nidulans (Piotrowska 1980). The genes coding for the main trans-acting factor mediating this control mechanism are identified in S. cerevisiae (aCN4) and N. crassa (cpc-1). These genes encode bZIP-type transcriptional activators which bind to palindromic general control recognition elements (GCRE's, consensus sequence 5' ATGA(C/G)TCAT 3'; Hope and Struhl, 1985, Arndt and Fink 1986; Ebbole et al., 1991) in the promoter sequence of many amino acid biosynthetic genes and stimulate their transcription. Here we report the cloning and initial characterization of OcA, the GCN4/CPC-1 homologue of the filamentous fungus Aspergillus niger.

abstract No: 


Full conference title: 

    • ECFG 3rd (1996)