The filamentous fungus Aspergillus nidulans is known to produce both a carcinogenic mycotoxin, sterigmatocystin, and an antibiotic, penicillin, as secondary metabolites. Previous work in our lab has indicated that production of both metabolites is dependent on the activity of G(i)a subunit of a heterotrimeric G protein. To investigate the involvement of the catalytic subunit of cAMP-dependent protein kinase (PkaC), a likely downstream target of G(i)a activity, in the production of these natural compounds, we have initiated the genetic characterization of this protein. We screened an A. nidulans cosmid library with the EST clone n8d03al.rl, predicted to encode the PkaC, and obtained two overlapping cosmids, pWE08C4 and pWE06A11. Southern analysis showed that the putative pkaC gene is located on a 5.4 kb HindIII fragment. Sequence analysis of this region revealed that the pkaC gene is highly conserved among fungal species on both the nucleotide and the deduced amino acid sequences.
Fungal Genet. Newsl. 46 (Supl):
Full conference title:
Fungal Genetics Conference 20th
- Fungal Genetics Conference 20th (1999)