Novel antifungal agents are constantly required due to the build up of disease resistance in fungal pathogens and the toxic effects of existing agents. Targets must be identified in the pathogens which when disrupted have a debilitating effect on the fungi, inhibiting their ability to infect the host and cause disease. Map kinases have been found to be involved in hypo-osmolarity signal transduction and cell wall integrity in Saccharomyces cerevisiae and may therefore be putative antifungal targets. Using Aspergillus nidulans as a model, we have designed PCR primers based on consensus to the PKC protein kinase family. As a second strategy, osmotically sensitive mutants are being produced by UV mutagenesis which only grow on osmotically buffered medium. The long term strategy is to clone the full length genes and to study the effect of knockouts on the phenotype of A. nidulans.
Fungal Genet. Newsl. 46 (Supl):
Full conference title:
Fungal Genetics Conference 20th
- Fungal Genetics Conference 20th (1999)