We have described two novel Fusarium transcription factors: ftf1 and ftf2, wich show a high nucleotide and aminoacidic homology but very different genomic architecture and expression. Both genes contain an ORF of 3240 nt including a 49 bp intron. The predicted 1080 amino acid protein contains a Zn(II)-Cys6 binuclear cluster DNA-binding motif with homology to various fungal regulatory proteins. The gene ftf1 is specifically expressed on early stages of infection, while ftf2 shows a very low level of expression in vivo and in vitro. We have analyzed the presence and expression of both genes in 14 formae speciales of F. oxysporum. The results mostly confirm those previously obtained in F. oxysporum f.sp. phaseoli. Chromosome hibridizations show the presence of ftf2 in all the formae speciales and several copies of ftf1 in different chromosomes. We have also analyzed the presence of transposon marsu and the homologue of bimB3 (Aspergillus nidulans). RT-PCR analyses of expression using primers specific for each gene show that ftf2 is expressed in vitro in all the formae speciales.
Full conference title:
23rd Fungal Genetics Conference
- Fungal Genetics Conference 23rd (2002)