The authors isolated the telomere sequences at the chromosomal terminal regions of Aspergillus oryzae, and showed that their structure was a repeated sequence constituted of 12bp repeat unit (TTAGGGTCAACA) (AMB61: 247, 2003). As telomere is participated in the replication of the chromosomal DNA and its stabilization of their ends, development of new type of the replicating vectors is expected. The replicating function of the telomeres of A. oryzae and the conservation of the chromosomal ends by them are not investigated yet. In this study, we constructed the two types of the telomere-attached vectors to examine their function. The vector I was constituted of single copy of TEL134, a clone of A. oryzae telomere (TTAGGGTCAACA)10 with its associated sequence, inserted into a vector of a integrated type, pPTRI (ptrA as a marker gene, TaKaRa Bio, JAPAN). The vector II was constituted of two copies of TEL134 inserted into pPTRI, where the two TEL134 were inserted as inverted repeat. A 0.8 kb DNA fragment derived from a vector for Escherichia coli was put as a spacer sequence between those TEL134 DNA. Namely, the spacer sequence was positioned as vector-(TTAGGGTCAACA)10-spacer-(TGTTGACCCTAA)10-vector. A. oryzae NFRI1599 were transformed with those telomere-attached vectors and pPTRI as a control plasmid. We are going to characterize the structure of the introduced vectors and the transformants.
Full conference title:
23rd Fungal Genetics Conference
- Fungal Genetics Conference 23rd (2002)