In fungi, environmental signals can be transduced into intracellular responses by the action of MAP kinase cascades, which are similar to the ones found in the mammalian cells. Mitogen-activated protein kinases (MAPK) are a family of serine/threonine protein kinases widely conserved among eukaryotes and are involved in many essential developmental processes such as mating, sporulation and pathogenicity. In this research work we are studying the role of mpkB, an MAP kinase gene found in the model filamentous fungus Aspergillus nidulans. The mpkB gene is a homologue of the FUS3/KSS1 MAP-kinase genes in the yeast Saccharomyces cerevisiae. FUS3 regulates mating in response to pheromones in haploid yeast cells and KSS1 regulates filamentous growth in response to nitrogen limitation in diploid yeast cells. In order to elucidate the role of mpkB in development and secondary metabolism in A. nidulans, we have generated a mpkB disruption strain. Loss of MPKB function results in a blockage in biosynthesis of the mycotoxin sterigmatocystin.
Full conference title:
23rd Fungal Genetics Conference
- Fungal Genetics Conference 23rd (2002)