As a part of a series of studies regarding the microbial biota in manned space environments, we isolated fungi from six pieces of equipment recovered from the Japanese Experimental Module "KIBO" of the International Space Station (ISS) and from a Space Shuttle. Thirty-seven strains of fungi were isolated and identified, and were investigated with regard to morphological phenotypes and antifungal susceptibilities. The variety of fungi isolated in this study was similar to several previous reports. The dominant species belonged to the genera Penicillium, Aspergillus, and Cladosporium, which are potential causative agents of allergy and opportunistic infections. The morphological phenotypes and antifungal susceptibilities of the strains isolated from space environments were not significantly different from those of reference strains on Earth.