Caspofungin and Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Enhance Neutrophil Antifungal Functions against Candida albicans and Candida dubliniensis Pseudohyphae.

C. GIL-LAMAIGNERE1, A. GRESS1, E. ROILIDES2, F.M.C. MÜLLER1

Author address: 

1 Dept. of Pediatrics, Würzburg, Germany, 2 Aristotle Univ., Thessaloniki, Greece

Abstract: 

Background: Caspofungin (CS) is a member of a new class of potent antifungal drugs, the candins, currently under development. Its effects on the antifungal activity of untreated or GM-CSF pre-treated neutrophils (PMN) against pseudohyphae ofCandida albicans (CA) and Candida dubliniensis (CD) were assessed. Methods: After purification, PMN were pre-incubated in RPMI supplemented with 10% autologous serum in presence or absence of 100 ng/ml GM-CSF for 3 h. Pseudohyphae were incubated ± 5 ng/ml CS and/or GM-CSF pre-treated or untreated PMN for 2 h. Damage of pseudohyphae was measured by XTT colorimetric assay. Results: The mean±SE % hyphal damage induced by CS, untreated PMN (U-PMN) and/or GM-CSF pre-treated PMN (GM-PMN), at effector:target ratio of 1:4, on CA (n=6) or CD (n=7) pseudohyphae is shown below. see table Conclusions: 1) CS had an additive effect on PMN-induced hyphal damage. 2) Pre-treatment of PMN with GM-CSF enhanced their antifungal functions against Candida pseudohyphae. 3) The combination of CS with GM-CSF pre-treated PMN displayed superior effects than its combination with untreated PMN. 4) The additive effects of GM-CSF, PMN and CS need to be further evaluated in animal models and clinical trials, since they may have important implications in the management of candidiasis
2002

abstract No: 

M-865

Full conference title: 

NULL
    • ICAAC 42nd