Candida albicans confers ampicillin resistance to Streptococcus gordonii in co-cultures

A. Maddi, 14214, L. M. Yerke, R. Mancuso, 14214, C. Jennifer, N. Sadhak

Author address: 

State Univ. of new York, Buffalo, NY

Abstract: 

Background: Oral biofilms are complex and contain various microorganisms including bacteria and fungi. The pioneer oral plaque bacteria, Streptococcus gordonii and the most abundant oral pathogenic fungus, Candida albicans are known to interact with each other. Past studies have shown that S. gordonii binds to hyphae of C. albicans in a corncob like structure. However, the exact mechanisms and outcomes of their interactions are not clearly understood. We hypothesized that C. albicans and S. gordonii confer antimicrobial resistance to one another when grown in co-cultures. Methods: C. albicans and S. gordonii were grown in a specially prepared synthetic minimal media [Tryptic Soy Broth Yeast Extract (TSBY) + Yeast Nitrogen Base (YNB)] with and without the antibacterial agent, ampicillin, for 2, 4, 6 and 8 hrs. The cultures were then filtered to eliminate C. albicans and the resulting filtrate was then plated on TSBY agar and S. gordonii colonies were counted. Similarly C. albicans and S. gordonii were also grown in co-cultures with caspofungin (antifungal agent) for 2, 4, 6 and 8 hrs. These cultures were plated on YNB agar with ampicillin and the resulting C. albicans colonies were counted. Proteomic analysis of secreted protein resulting from the co-cultures was performed using SDS PAGE analysis, silver staining and mass spectrophotometric analysis. Results: The results from the antibiotic testing showed that S. gordonii was able to grow significantly greater (p
2016

abstract No: 

LB-004

Full conference title: 

ASM Microbe 2016
    • ASM microbe 1st (2016)