Aspergillus fumigatus is an important pathogen of the immunocompromised host, causing pneumonia and invasive disseminated disease with high mortality rates. We have cloned three conserved components of the cAMP signal transduction cascade, the adenylate cyclase (acyA), a Ga subunit (gpaB) and a catalytic subunit of protein kinase A (pkaC). Deletion of the genes revealed, that all components influence growth, development and sporulation of A. fumigatus. Conidia of acyA and gpaB deletion mutants were killed more efficiently in a macrophage assay. Furthermore, a possible cAMP-dependent regulation of the A. fumigatus virulence determinant pksP, which encodes a polyketide synthase, was investigated by constructing a pksP-promoter lacZ-fusion. pksP-expression was significantly reduced in gpaB deletion backround compared with the expression of the gene fusion in a wild-type strain. We also started to investigate the cAMP-dependent pksP-regulation by promoter mutation analysis, in order to fully understand the role of PKSP during infection.
Fungal Genet. Newsl. 50 (Supl):abstract
Full conference title:
22nd Fungal Genetics Conference
- Fungal Genetics Conference 22nd (2001)